Table 1.
Kinetic parameters for Nav1.5 with or without WT Cav3 and with three Cav3 mutants
| pcDNA3 | Cav3 | V14L | T78M | T79R | |
|---|---|---|---|---|---|
| INa density | |||||
| pA/pF | −330±96 | −439±74 | −506±74 | −351 ±42 | −451±33 |
| Activation | |||||
| V½, mV | −44±1 | −50±5 | −53±6 | −40±1 | −51±8 |
| Slope factor | 4 | 4 | 4 | 4 | 4 |
| Inactivation | |||||
| V½, mV | −83±1 | −85±1 | −90±4 | −84 ±4 | −86±3 |
| Recovery | |||||
| ιf, ms | 2.3±0.2 | 2.7±0.2 | 3.0±0.6 | 3.1±1.3 | 3.1±0.4 |
| ιs, ms | 49±4 | 51±4 | 50±8 | 47±16 | 56±12 |
| Af | 79%±2 | 76%±1 | 76%±2 | 77 %±5 | 81%±4 |
| n | 8 | 6 | 6 | 6 | 4 |
Table 1. The fitted kinetic parameters and INa density from n experiments were averaged and are reported as means ± SD. pcDNA3(An expression vector), WT Caveolin3 (Cav3) and three Cav3 mutants (V14L, T78M and T79R) were transiently expressed in a mammalian cell line stably expressing SCN5A. All parameters were analyzed by one-way ANOVA across pcDNA3, WT Cav3 and three Cav3 mutants. There is no statistically significant for INa density, activation, inactivation from recovery and rate of recovery when pcDNA3, WT Cav3 or the three Cav3 mutants was expressed in NaV1.5 stable cell lines.