Table 2.
The number of mutations in subclone 18.6
| Subclone 18.6 | Mutation no. |
|---|---|
| 18.6.1 | 2 |
| 18.6.2 | 0 |
| 18.6.3 | 3 |
| 18.6.4 | 1 |
| 18.6.5 | 2 |
| 18.6.6 | 0 |
Each clone was grown for 23.41 cell generations from founder. Six clones were analyzed, 2000 bp containing 1225 G or C nucleotides were sequenced, or a total of 7350 G or C base pairs. Estimation of the average frequency of mutations per base pair was done according to the Poisson distribution, the zero order value was − ln(7342/7350) = − ln 0.9989 = 1.089 × 10−4. The mutation rate was 4.65 × 10−5 mut/bp per cell generation. According to the Taylor series, the average frequency was 1.1 × 10−3 which yielded a mutation rate of 4.69 × 10−5 mut/bp per cell generation.