Table 3.

The number of mutations in progeny of clone 6

Clone 6	Mutation no.
6.2	0
6.3	2
6.4	0
6.5	0
6.6	0
6.7	1
6.8	0
6.9	0
6.10	1

Each clone was grown for 28.71 cell generations from founder. Nine clones were analyzed, 2000 bp containing 1225 G or C nucleotides were sequenced, or a total of 11 025 G or C base pairs. Estimation of the average frequency of mutations per base pair was done according to the Poisson distribution, the zero-order value was − ln(11 021/11 025) = −ln 0.9996 = 3.63 × 10⁻⁴. The mutation rate was 1.26 × 10⁻⁵ mut/bp per cell generation. According to the Taylor series, the average frequency was 0.4 × 10⁻³ which yielded a mutation rate of 1.39 × 10⁻⁵ mut/bp per cell generation.