Table 4.
The number of mutations in the progeny of a subclone of clone 18
| Subclone 18.6 | Mutation no. |
|---|---|
| 18.6.1 | 0 |
| 18.6.2 | 0 |
| 18.6.3 | 0 |
| 18.6.4 | 0 |
| 18.6.5 | 2 |
| 18.6.6 | 0 |
Each clone was grown for 23.41 cell generations from founder. Six clones were analyzed, 300 bp sequenced comprehending 176 G or C containing nucleotides, or a total of 1056 G or C base pairs. Estimation of the average frequency of mutations per base pair was done according to the Poisson distribution, the zero-order value was − ln(1054/1056) = −ln 0.998 = 1.90 × 10−3. The mutation rate was 8.1 × 10−5 mut/bp per cell generation. According to the Taylor series, the average frequency was 2 × 10−3 which yielded a mutation rate of 8.54 × 10−5 mut/bp per cell generation.