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. 2009 Dec 7;38(5):e29. doi: 10.1093/nar/gkp1138

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — The scheme shows the sequences of two FRET probes and two PCR primers (forward and reverse) used in the Taqman assays aligned with a detected sequence of 96-mer target oligodeoxyribonucleotide. Also shown are the forward primers used in the Snake assays. These primers comprise the regular primer sequence and 8–14-mer 5′-flap sequences. The segments of the flaps that participate in the intramolecular stem formation of the folded Snake amplicons and their binding site within the target sequence are underlined. FAM is 6-fluorescein and Q is a BHQ1 quencher.