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. 2009 Dec 8;38(5):e32. doi: 10.1093/nar/gkp1140

Figure 2.

Figure 2.

Transgene expression upon coupling to kis. (A) eGFP in CHO wild-type cells. CHO wild-type cells were cotransfected with the eGFP expression construct and pAG60. G418 resistant cell pools were generated and evaluated for eGFP expression 20 days after transfection. (B) eGFP expression from a kis-coupled expression construct in kid expressing cells The bicistronic eGFP expression construct coupled to kis in pGNKis was cotransfected together with the neomycin-resistance gene into the two kid-regulated cell clones #14 (upper) and #18 (lower). The cells were cultivated with or without Dox for the indicated period in presence of G418 and puromycin. The eGFP expression profile of the cultures was determined by FACS analysis. The numbers above the bars indicate the percentage of eGFP-expressing cells in the respective cell pool. (C) eGFP expression from selected cell pools in absence of selection pressure. Clone #14 and 18 derived eGFP expressing pools generated upon 52 days of cultivation in absence of Dox and presence of G418 and puromycin were subjected to cultivation in media without Dox and any selection drugs. Expression was measured after 28 days of further cultivation.