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. 2009 Dec 9;38(5):1723–1737. doi: 10.1093/nar/gkp1144

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Nuclease assay to test for inhibition of EcoKI by ArdB proteins. Lane 1, undigested DNA; lane 2, EcoKI digested DNA; lane 3, plus ocr; lane 4, plus Orf18 ArdA; lane 5, plus ArdB_YAF; lane 6, plus ArdB_YFJ; lane 7, plus KlcA₁₃₆; lane 8, plus ArdB_CFT; lane M, DNA ladder with sizes in kb. Plasmid DNA used was non-methylated pBRsk1. Ratio of the anti-restriction proteins (monomers) to EcoKI was 20:1. In each case the reaction mixture minus DNA was made up and incubated for 2 min at room temperature. The reaction was then initiated by adding DNA. Digestion was carried out for 8 min at 37°C before quenching at 68°C for 10 min. CC, closed circular plasmid; L, linear plasmid; OC, open circular plasmid.