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. 2009 Dec 14;38(5):1489–1503. doi: 10.1093/nar/gkp1149

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Aprataxin binds to a conserved di-phosphorylated CK2 motif in MDC1. (A) MDC1 contains six conserved motifs with a core SDTD motif that is di-phosphorylated in vivo (48–50). (B) ITC binding isotherms for interaction of wild-type aprataxin FHA with pSDTD and SDpTD phosphopeptides (C, D) ITC binding isotherms for the titration of pSDpTD phosphopeptide into wild-type and K38A aprataxin FHA, respectively. (E) Diagram of GFP-MDC1 mutants used in the pull-down assays. (F) Aprataxin FHA GST pull downs from HeLa cells expressing wild-type GFP-MDC1, a mutant containing a deletion of the SDTD region (ΔSDTD), and a mutant where the Thr residues contained in the SDTD sequence were replaced by Ala (SDAD)₂.