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The pH dependency of the functionality transfer reaction. The transfer reactions were performed using 1.5 µM of S-functionalized ODN1F [GS(Me,Ph)] and 1.0 µM of the target ORN5 (G) in 50 mM phosphate or carbonate buffer (pH > 9) containing 100 mM NaCl at 25°C for 1 h, followed by HPLC.
