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Selectivity to the guanine base in transfer reaction of the pyrene derivative. The transfer reactions were performed in 50 mM carbonate buffer containing 100 mM NaCl at pH 9.6 for 1 h, followed by HPLC monitored by fluorescence at 518 nm with emission at 494 nm. Using 1.5 µM of S-functionalized ODN1F [GS(Me, Pyrene)] and 1.0 µM of the target ORN5 (Y).
