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Comparison of the reactivity of the purine analogs. (A) Reaction at pH 9.6. (B) Reaction at pH 7.0. The transfer reactions were performed using 30 µM of S-functionalized ODN1F [GS(Me,Ph)] and 25 µM of the target ODN6 in 50 mM carbonate buffer containing 100 mM NaCl followed by HPLC. Abbreviations of nucleobase in the graph represent 2-AP for 2-aminopurine, 6-AP for 6-aminopurine (adenine), G for guanine and Hx for hypoxanthine.
