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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: J Cell Biochem. 2010 Mar 1;109(4):672–682. doi: 10.1002/jcb.22462

Figure 5. Phosphorylated SMAD1,5,8 Immunofluorescence.

Figure 5

Osteoclasts were differentiated by RANKL for 1, 3 or 5 days and subjected to immunofluorescence staining against phosphorylated SMAD1,5,8 (green). Cells were costained against α-tubulin (red) and with DAPI (dark blue) to show cell outline and nuclei, respectively. Parallel slides from each day were stained without P-SMAD antibody as a negative control (bottom row and not shown). All images are at equal magnification and were acquired and processed identically.