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Colocaliztion of eGFP–syntenin and mouse syndecan-2 in CHO cells. Transient CHO transfectants expressing wild-type mouse syndecan-2 (A, A′, B, and B′), C9-mouse syndecan-2 (C and C′), or C30-mouse syndecan-2 (D and D′) were cotransfected with plasmids encoding eGFP (A and A′) or eGFP-syntenin fusion protein (B–D and B′–D′). Mouse syndecan-2 ectodomain was detected with mAb 10H4 (A′–D′) and Texas-X-Red-labeled second antibody.
