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. Author manuscript; available in PMC: 2010 Mar 11.
Published in final edited form as: Open Immunol J. 2009 Jan 1;2:163–167. doi: 10.2174/1874226200902010163

Figure 1. Eosinophils generated in cultures of unselected bone marrow from both BALB/c and C57BL/6 mice.

Figure 1

(A) Bone marrow cultures were initiated at 107 cells/mL. The number of viable cells was determined at timepoints shown in response to rmSCF (100 ng/mL) and rmFLT-3L (100 ng/mL) for 4 days after isolation, followed by rmIL-5 (10 ng/mL) alone thereafter. (B) Eosinophils were identified at day 10 in culture by side scatter (SSC, y-axis) and reactivity with anti-Siglec F+ antibodies (x-axis).