Fig. 2.

Time-lapse video microscopy images of amyloid plaque formation. (A) Schematic representation of the mechanism of amyloid plaque. Hypothetical pathway (gray background) of amyloid plaque formation and pathway consistent with presented data (white background). (B) THP-1 cells were incubated with 60 μg/mL Aβ(1–40), containing 10% soluble ⁴⁸⁸Aβ and monitored with fluorescence (Top) and phase contrast (Bottom) microscopy. Each well was supplemented 100 ng/mL seeds, corresponding to 0.2% of the total amount of Aβ peptide added on day zero. Beginning of amyloid plaque formation is indicated by arrows. Ellipses are drawn to guide the eye.