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. 2010 Jan 11;107(5):1870–1875. doi: 10.1073/pnas.0909350107

Fig. 2.

Fig. 2.

Si NWs can deliver and codeliver diverse biomolecular species to a broad range of cell types. (A)–(D) Various immortalized and primary cell types expressing fluorescent proteins after being transfected with plasmid DNA via the Si NWs: (A) HeLa S3 cells (pCMV-TurboRFP), (B) human fibroblasts (pCMV-TurboRFP), (C) NPCs (pCMV-mCherry), and (D) primary rat hippocampal neurons (pCMV-dTomato). Scale bar in A–D, 25 μm. (E)–(H) Human fibroblasts transfected with a variety of biochemicals. To verify intracellular delivery, following a 24-h incubation on coated Si NWs, cells were replated on glass coverslips for imaging. Epifluorescence images show > 95% of the cells receive: (E) siRNA (Alexa Fluor 546 labeled AllStar Negative Control siRNA), (F) peptides (rhodamine labeled 9-mer), (G) plasmid DNA (labeled with Label-IT Cy5), and (H) proteins (recombinant TurboRFP-mito). Scale bars in E–H, 50 μm. (I)–(L) Fluorescence images of human fibroblasts cotransfected with two different molecules using Si NWs: (I) two different siRNAs (Alexa 546 labeled siRNA—yellow & Alexa 647 labeled siRNA—magenta), (J) a peptide and an siRNA (rhodamine labeled peptide—yellow & Alexa 647 labeled siRNA—magenta), (K) plasmid DNA and an siRNA (Cy5-labeled TagBFP plasmid DNA—yellow & Alexa 546 labeled siRNA—magenta), and (L) a recombinant protein and an siRNA (recombinant TurboRFP-mito—yellow & Alexa 647 labeled siRNA—magenta). In E to L, to facilitate identification, cell membranes were labeled with fluorescein (Gray). Scale bars in I–L, 25 μm.