Fig. 5.
Cu-free click chemistry and Staudinger ligation products are observed in a variety of tissues in vivo. Mice were injected with Ac4ManNAz (300 mg/kg, i.p., +Az) or vehicle (70% DMSO,-Az) once daily for 7 d. On day 8, the mice were injected with either (A) ALO-FLAG (0.8 mmol/kg, i.p.), blot was exposed overnight; (B) DIMAC-FLAG (0.8 mmol/kg, i.p.), blot was exposed for 5 min; (C) DIFO-FLAG (0.16 mmol/kg, i.p.), blot was exposed for 1 h; or (D) PHOS-FLAG (0.8 mmol/kg, i.p.), blot was exposed for 10 s. Three h postinjection of the FLAG conjugates, the mice were euthanized, and organs (liver, heart, and intestines) were harvested and homogenized. The organ lysates were analyzed by Western blot probing with HRP-anti-FLAG. Each lane represents organ lysate from a single representative mouse.