Fig. 3.

Probiotics induce CD11c⁺ rDCs. (A) CD11c⁺ DCs isolated from MLN of Cont or Pro group mice were stimulated with PMA/ionomycin. The levels of immunoregulatory molecules were measured by real-time PCR. (B) CD11c⁺ DCs from each group were cocultured with Do11.10 CD4⁺ T cells labeled with CFSE and ovalbumin peptide (0.3 μg/mL) in the absence (−) or presence (+) of exogenous TGF-β. (C) Levels of Foxp3⁺CD25⁻ and Foxp3⁺CD25⁺ populations were measured by FACS. (D) MLN CD11c⁺ DCs from IRT5-fed mice were cocultured with CD4⁺ T cells in the presence of ovalbumin peptide and inhibitors for rDCs. w/o, without. After coculture, alteration of the Foxp3⁺ population was measured by FACS. Data are from 10 mice per group and are the average of three independent experiments.