Fig. 7.

Increased chemoattractant expression leads to enrichment of Tregs in the inflamed region. The expression levels of chemokines in MHC class II-positive antigen-presenting cells (A) and their receptors in CD4⁺ T cells (B) were analyzed by quantitative real-time PCR. (C) CFSE-labeled T cells obtained from MLN of Cont and Pro groups were adoptively transferred to AD mice. The migration of CFSE⁺(CD4⁺)Foxp3⁺ Tregs to the ear of AD mice was analyzed by IHC. The arrows indicate CFSE⁺(CD4⁺)Foxp3⁺ Tregs. Data are from 10 mice per group; error bars indicate SD. Data are representative of three independent experiments. *P < 0.05; **P < 0.005; ***P < 0.001.