Fig. 3.
Expression of the LHRcAMP− and LHRLH− BAC transgenes in male mice. (A) Expression of mRNA of the transgenes (LHRcAMP− and LHRLH−) in different tissues of TG mice as analyzed by RT-PCR, showing strong specific expression in gonads (product sizes 559 and 960 bp, respectively) and weak expression in brain. (B) Specific [125I]-hCG binding to testis homogenates of WT, LuRKO, LHRcAMP−, and LHRLH− mice (n = three per group), as well as, in a separate binding assay, WT (n = 4) and LHRLH−/cAMP− (n = 2) mice (C). Each bar is the mean + SD. Different letters above the bars in B indicate that these levels differ significantly (P at least <0.05).