Fig. 4.

Gonadal and genital phenotypes: macroscopic and microscopic appearance of the WT and mutant male mice. (Top) Testes and accessory sex organs (from left to right) of LuRKO mice (A) and mice expressing in the LuRKO background LHR^LH− (B), LHR^cAMP− (C), both transgenes (LHR^{LH−/ cAMP−}) (D), and WT mice (E). (Middle) Immunofluorescence of the reporter genes (eCFP, green, corresponding to LHR^LH−; RFP, red, corresponding to LHR^cAMP−) specifically expressed in Leydig cells (LC). (Bottom) Merged pictures of the two reporter genes, showing coexpression of the two transgenes in LC of LHR^{LH−/cAMP−} mice. (Scale bars: Top, 1 cm; Middle and Bottom, 25 μm).