Fig. 2.

Regenerative delay. Representative anatomical coronal MRI of the same 9-month-old control (A) and Mdr2^−/− (KO) (B) mice before and on days 4 and 7 following PHx. Dashed green lines encircle the liver, and yellow dotted lines encircle the residue of the resected lobe. (Scale bar: 1 cm.) Liver volume monitoring after 70% PHx of 3-month-old (n = 8 per time point per group) (C) and 9-month-old (n ≥ 10 per time point per group) (D) in control (blue) and KO (green) mice. Percentage of liver volume compared with the volume before surgery was calculated for each animal, and averages ± SD were plotted. *P < 0.01; **P < 0.05. The horizontal dashed line marks liver volume restoration. Representative liver sections of control and KO 9-month-old mice killed on day 6 after 35% PHx immunostained for BrdU (E) and CDC47 (F). Quantification of the BrdU- and CDC47-positive cells (E and F, respectively) was done at the indicated time points for 30 randomly selected fields (n ≥ 3 mice per time point per group). HPF, high-power field.