Fig. 5.

Membrane insertion assayed based on glycosylation in microsomes. (A Top) setup for membrane insertion assay with a LepP2 domain and two glycosylation sites flanking S4. Middle and bottom panels: SDS/PAGE gels of in vitro transcribed/translated products and the corresponding calculated insertion efficiencies. (B Upper) Setup for the membrane insertion assay with two glycosylation sites upstream of S4s. (Middle and Bottom) SDS-gels of in vitro transcribed/translated products and the corresponding calculated insertion efficiencies. Data represents the average of three independent experiments with standard deviations.