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Fixation reduces distances between subtelomeres. Box plots for 3D distances between Tel6R andTel4R (short–long arm), labeled in green and red, respectively. Two independent experiments both in living cells (Left) and fixed cells (Right) are shown. The median is represented by a dark line, the box represents the interquartile range, and whiskers the data range (except for outliers, shown as open circles). N, number of cells analyzed.
