Fig. 3.

Arm length influences localization of subtelomeres at the nuclear periphery. (A) 2D localization probability maps for different subtelomeres. Top and bottom halves are mirrored (around the green dashed line) for visual convenience. Yellow circle and red dotted outline represent a “median” nuclear envelope and nucleolus, respectively. The probability to find the locus inside different regions of the nucleus is indicated by the percentage of the enclosing contour. Hot colors indicate higher probability densities. Chromosome arm sizes are indicated on the right (kb). N, number of cells analyzed. (B) Median polar angle vs. chromosome arm length, annotated with the subtelomere label. Dashed vertical line represents the change point value, gray area represents its 95% CI. Linear regression relationships are Y (°) = 46.3 + 6.1 10⁻² × L (kb), for arm length L ≤ 430 kb and Y (°) = 85.5–1.9 10⁻² × L (kb), for L > 430 kb. (C) Median subtelomere–SPB distances calculated as dist(Tel, SPB) = sqrt(R_Tel² + R_SPB² + 2 R_Tel R_Tel cos α), where R_Tel is the distance of subtelomere to the nuclear center, R_SPB is the distance of SPB from the nuclear center = 900 nm, and α is the angle with the horizontal axis. Dashed vertical and gray areas are defined as in B.