Fig. 4.

SPB–centromere link influences subtelomere–subtelomere distances, whereas nucleolar volume limits subtelomere positioning and increases subtelomere–subtelomere distances. (A) Localization probability maps for subtelomeres from a short arm (Tel9R), a middle arm (Tel10R), and a long arm (Tel4R). The size in kilobases of the respective arms is indicated between brackets. Maps are displayed as in Fig. 3. (B) Box plots (as in Fig. 2) for pairwise distances in the absence or presence of nocodazole (+noco) for subtelomere pairs Tel6R-Tel3L (short–short) and Tel6R-Tel4R (short–long). (C) Probability maps for subtelomeres of short arms (left) or long arms (right). Cells were either grown without rapamycin (control) or in the presence of rapamycin (+rapa) for 20 min. The size of the nucleolus is reduced in the presence of rapamycin (quantified in Table S4). (D) Median polar angle vs. chromosome arm length, as in Fig. 3. Median polar angles in the absence (red triangles) or presence (black circles) of rapamycin plotted as a function of chromosome arm size. A change point of similar value and CI is found in both conditions. (E) Box plots for pairwise distances in the absence or presence of rapamycin (+rapa) for subtelomere pairs Tel6R-Tel3L (short–short) and Tel6R-Tel4R (short–long).