Table 1.
Dynamic analyses of pair-wise subtelomere distances
| Parameter | 6R-2loci | 16R-URA3 | 6R-3L (S–S) | 4R-13R (L–L) | 4R-15R (L–L) | 6R-4R (S–L) |
| Total run length | ||||||
| Frames | ||||||
| Mean | 35.5 | 5.3 | 8.7 | 7.3 | 7.9 | 3.3 |
| SD | 5.2 | 7.5 | 9.9 | 9.1 | 9.6 | 6.1 |
| Seconds | ||||||
| Mean | 287.7 | 42.8 | 70.3 | 59.3 | 63.7 | 26.4 |
| SD | 42.3 | 60.4 | 80.2 | 73.9 | 77.7 | 49.8 |
| Maximum run length | ||||||
| Frames | ||||||
| Mean | 24.3 | 2.3 | 3.9 | 2.9 | 3.7 | 1.5 |
| SD | 10.9 | 3.5 | 5.2 | 3.9 | 5.1 | 2.9 |
| Seconds | ||||||
| Mean | 196.8 | 18.6 | 31.5 | 23.8 | 29.6 | 12.4 |
| SD | 88.7 | 28 | 42.3 | 31.4 | 41.5 | 23.7 |
Cells were visualized for 5.4 min in the two channels (100-ms illumination per frame, 40 consecutive stacks). Arrays of lac and tet operators were inserted 15.7 kb apart (6R-2loci), between a subtelomere and a central region (16R-URA3) or between two subtelomeres from short (S) or long (L) chromosome arms. 4R, 15R contain Y’ sequences. A run is defined as a set of consecutive frames for which the distance between two labeled loci is <600 nm. The number of runs during the duration of the experiment indicates the frequency of a possible interaction, whereas stability is indicated by the maximum run length.