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Esophageal innervation is influenced by increased GDNF. A, NADPH-d myenteric neurons in WT esophagus or from PBS-injected mice are typically found in small clusters with a few small neuronal fibers between ganglia. B, GFAP–Gdnf mice had more esophageal neurons and a dramatic accumulation of neuronal fibers near enteric ganglia. GDNF-injected mice (C) and Myo–Gdnf (D) had more abundant neurons, as well as a higher density of neuronal fibers. Scale bar, 100 μm.
