Figure 8.

GFAP–Gdnf mice have increased neuronal fiber density near enteric glia. A, D, GFAP immunohistochemistry in WT and GFAP–Gdnf mice. B, E, The same region of the bowel was simultaneously stained using NADPH-d histochemistry. C, F, For these merged images, NADPH-d cells and fibers are pseudo-colored green to facilitate combining fluorescent (A, D) and bright-field (B, E) images and to provide increased contrast to the GFAP immunohistochemistry. Thick nerve fiber bundles accumulate near GFAP-expressing cells in the transgenic mice. Arrows highlight corresponding areas of GFAP and NADPH-d in images. Scale bar, 100 μm.