Table 2.
Quantitative cell size data
| Cell size (μm2) | Fold change | p value | ||
|---|---|---|---|---|
| WT |
GFAP–Gdnf mice |
|||
| A, Excess GDNF increases cell size of some mature submucosal and myenteric neurons in small bowel and colon> | ||||
| Small bowel myenteric Cuprolinic | 206.5 ± 9.3 | 260.8 ± 9.6 | 1.26 | <0.001 |
| Colon myenteric Cuprolinic | 213.1 ± 8.7 | 229.3 ± 7.1 | 1.07 | 0.028 |
| Small bowel myenteric NADPH-d | 170.5 ± 4.4 | 202.7 ± 6.2 | 1.18 | <0.001 |
| Colon myenteric NADPH-d | 192.9 ± 5.6 | 245.4 ± 7.2 | 1.27 | <0.001 |
| Small bowel myenteric TH | 1099.7 ± 158.7 | 1107 ± 132.1 | 0.261 | |
| Small bowel submucosal acetylcholinesterase | 254.5 ± 6.9 | 269.7 ± 7.9 | 0.181 | |
| Colon submucosal acetylcholinesterase | 451.1 ± 17.0 | 453.9 ± 16.1 | 0.909 | |
| PBS-injected mice |
GDNF-injected mice |
|||
| Small bowel myenteric Cuprolinic | 203.7 ± 6 | 241.5 ± 7 | 1.19 | <0.001 |
| Colon myenteric Cuprolinic | 214.3 ± 6 | 245.6 ± 7 | 1.14 | 0.002 |
| Small bowel myenteric NADPH-d | 183.4 ± 6 | 229.8 ± 6 | 1.25 | <0.001 |
| Colon myenteric NADPH-d | 203.1 ± 4 | 233.7 ± 5 | 1.15 | <0.001 |
| Small bowel submucosal acetylcholinesterase | 243.5 ± 6 | 265.7 ± 6 | 1.09 | 0.002 |
| Colon submucosal acetylcholinesterase | 396.7 ± 14 | 420.4 ± 14 | 0.142 | |
| WT |
GFAP–Gdnf mice |
|||
| B, Excess GDNF increases cell size of esophageal neurons | ||||
| Esophagus myenteric NADPH-d | 214.0 ± 9.8 | 318.3 ± 9.9 | 1.49 | <0.001 |
| PBS-injected mice |
GDNF-injected mice |
|||
| Esophagus myenteric NADPH-d | 181.5 ± 6 | 311.8 ± 7 | 1.72 | <0.001 |
| WT |
Myo–Gdnf mice |
|||
| C, GDNF trangene in skeletal muscle does not increase cell size of mature enteric neurons | ||||
| Esophagus myenteric NADPH-d | 238.9 ± 24 | 296.1 ± 29 | 0.199 | |
| Small bowel myenteric Cuprolinic | 182.1 ± 12 | 163.2 ± 25 | 0.531 | |
| Small bowel myenteric NADPH-d | 212.0 ± 15 | 237.2 ± 45 | 0.626 | |
| Small bowel submucosal acetylcholinesterase | 218.2 ± 9 | 214.0 ± 13 | 0.801 | |
| Colon myenteric Cuprolinic | 189.2 ± 24 | 176.4 ± 31 | 0.762 | |
| Colon submucosal acetylcholinesterase | 369.4 ± 32 | 324.9 ± 11 | 0.263 | |
The sizes of enteric neurons (±SEM) are shown for GFAP–Gdnf, Myo–Gdnf, and GDNF-injected mice. Because these mice are different ages and different strains, all comparisons are between animals with altered GDNF abundance and WT littermates. This table is organized to follow the text. The same data are also provided in supplemental Table 1 (available at www.jneurosci.org as supplemental material), organized by mouse genotype or treatment regimen. Animal numbers are the same as in Table 1.