Table 3.
Quantitative neuronal fiber density data
| Fiber density | Fold change | p value | ||
|---|---|---|---|---|
| WT |
GFAP–Gdnf mice |
|||
| A, GFAP–Gdnf transgenic mice versus WT | ||||
| Esophagus myenteric NADPH-d fiber bundles | 0.0 ± 0.0 | 1.96 ± 0.2 | ** | <0.001 |
| Esophagus myenteric NADPH-d single fibers | 1.94 ± 0.2 | 4.86 ± 0.6 | 2.52 | <0.001 |
| Esophagus submucosal NADPH-d single fibers | 35.8 ± 7.0 | 84.6 ± 14.8 | 2.36 | 0.018 |
| Small bowel myenteric NADPH-d fiber bundles | 3.2 ± 0.2 | 4.2 ± 0.2 | 1.31 | <0.001 |
| Small bowel myenteric NADPH-d single fibers | 38.6 ± 1.6 | 41.4 ± 1.4 | 0.162 | |
| Small bowel myenteric TH fiber bundles | 4.4 ± 0.1 | 4.4 ± 0.1 | 0.823 | |
| Small bowel myenteric TH single fibers | 12.2 ± 0.3 | 11.2 ± 0.4 | 0.92 | 0.035 |
| Small bowel myenteric ChAT fiber bundles | 4.4 ± 0.4 | 5.4 ± 0.2 | 1.23 | 0.02 |
| Small bowel myenteric ChAT single fibers | 10.6 ± 1.0 | 8.2 ± 1.2 | 0.07 | |
| Small bowel myenteric acetylcholinesterase fiber bundles | 4.0 ± 0.2 | 4.5 ± 0.2 | 1.13 | 0.04 |
| Small bowel myenteric acetylcholinesterase single fibers | 44.6 ± 1.2 | 46.4 ± 1.4 | 0.292 | |
| Colon myenteric NADPH-d fiber bundles | 4.0 ± 0.2 | 5.5 ± 0.2 | 1.37 | <0.001 |
| Colon myenteric NADPH-d single fibers | 51.0 ± 2.0 | 46.6 ± 1.2 | 0.059 | |
| Colon myenteric acetylcholinesterase fiber bundles | 5.2 ± 0.2 | 5.7 ± 0.2 | 0.108 | |
| Colon myenteric acetylcholinesterase single fibers | 45.4 ± 1.4 | 44.0 ± 1.0 | 0.448 | |
| PBS-injected mice |
GDNF-injected mice |
|||
| B, GDNF-injected mice versus PBS-injected controls | ||||
| Esophagus myenteric NADPH-d fiber bundles | 0.0 ± 0.0 | 4.8 ± 0.26 | ** | <0.001 |
| Esophagus myenteric NADPH-d single fibers | 4.0 ± 0.4 | 8.4 ± 0.6 | 2.07 | <0.001 |
| Small bowel myenteric NADPH-d fiber bundles | 3.6 ± 0.1 | 4.3 ± 0.1 | 1.19 | 0.002 |
| Small bowel myenteric NADPH-d single fibers | 47.8 ± 1.0 | 46.6 ± 0.6 | 0.228 | |
| Small bowel myenteric TH fiber bundles | 4.2 ± 0.1 | 4.1 ± 0.1 | 0.809 | |
| Small bowel myenteric TH single fibers | 14.1 ± 0.3 | 14.5 ± 0.4 | 0.431 | |
| Small bowel myenteric acetylcholinesterase fiber bundles | 4.7 ± 0.1 | 5.2 ± 0.2 | 1.11 | 0.018 |
| Small bowel myenteric acetylcholinesterase single fibers | 43.6 ± 0.8 | 45.4 ± 1.0 | 0.257 | |
| Colon myenteric NADPH-d fiber bundles | 6.7 ± 0.1 | 6.3 ± 0.1 | 0.94 | 0.031 |
| Colon myenteric NADPH-d single fibers | 41.2 ± 0.6 | 44.8 ± 0.8 | 1.09 | <0.001 |
| Colon myenteric acetylcholinesterase fiber bundles | 5.8 ± 0.2 | 6.1 ± 0.1 | 0.242 | |
| Colon myenteric acetylcholinesterase single fibers | 44.8 ± 0.8 | 45.8 ± 0.8 | 0.317 | |
| Colon myenteric TH fiber bundles | 6.0 ± 0.2 | 5.8 ± 0.2 | 0.337 | |
| Colon myenteric TH single fibers | 12.6 ± 0.2 | 12.0 ± 0.2 | 0.147 | |
| WT |
Myo–Gdnf mice |
|||
| C, Myo–Gdnf mice versus WT | ||||
| Esophagus myenteric NADPH-d single fibers | 7.8 ± 2.4 | 37.4 ± 4.5 | 4.79 | <0.001 |
| Esophagus submucosal NADPH-d single fibers | 22.6 ± 1.3 | 21.6 ± 1.3 | 0.598 | |
| Small bowel myenteric NADPH-d fiber bundles | 6.9 ± 0.5 | 7.5 ± 1.0 | 0.573 | |
| Small bowel myenteric NADPH-d single fibers | 45.4 ± 2.6 | 48.8 ± 2.6 | 0.385 | |
| Small bowel myenteric acetylcholinesterase fiber bundles | 9.9 ± 0.8 | 9.6 ± 1.0 | 0.801 | |
| Small bowel myenteric acetylcholinesterase single fibers | 45.0 ± 5.0 | 41.0 ± 4.2 | 0.584 | |
| Colon myenteric acetylcholinesterase fiber bundles | 12.8 ± 1.4 | 12.1 ± 1.7 | 0.779 | |
| Colon myenteric acetylcholinesterase single fibers | 37.6 ± 4.6 | 43.0 ± 3.2 | 0.387 | |
| WT |
Gdnf+/− |
|||
| D, Gdnf+/− mice versus WT | ||||
| Esophagus myenteric NADPH-d single fibers | 7.1 ± 1.4 | 2.6 ± 0.6 | 0.37 | 0.047 |
| Small bowel myenteric NADPH-d single fibers | 52.0 ± 2.0 | 42.4 ± 1.2 | 0.82 | 0.026 |
The number of neuronal fibers (±SEM) crossing the left or upper border of a 0.5 × 0.5 mm counting grid are shown. Fiber bundles and single fibers were counted separately. This table is organized to follow the text. The same data are also provided in supplemental Table 1 (available at www.jneurosci.org as supplemental material), organized by mouse genotype or treatment regimen. Animal numbers are the same as in Table 1.
**Cannot be calculated because of division by zero.