Fig. 3.
Deletion of the AT-rich region and CArG box from the endogenous telokin promoter specifically abolished telokin expression. An RNase protection assay was performed on RNA isolated from jejunum and bladder of wild-type (WT) and telokin knockout (KO) mice. The probe used (P) protects an ~255-bp fragment of the telokin mRNA and a 125-bp fragment of the MLCK mRNA. The positions of RNA size markers are indicated at right of the blot in base pairs. Either 2 or 10 μg of RNA were used in the assay, as indicated. Y, yeast RNA.