Figure 2.

Transgenic lines expressing Htr1a in the CeA and DG. (a) Tissue- and cell type–specific expression of Htr1a was achieved by making transgenic mice with the Htr1a coding sequences under the control of the Nrip2 promoter. (b–g) Crossing of transgenic founder lines to mice lacking the endogenous receptor (Htr1a^KO) resulted in receptor expression restricted to cells in the central nucleus of the amygdala (Htr1a^CeA; b,e) and granule cells of the dentate gyrus (Htr1a^DG; c,f), as detected by autoradiography using the Htr1a ligand ¹²⁵I-MPPI (dDG, dorsal DG; vDG, ventral DG). No receptor binding was detected in brains from Htr1a^KO mice (d,g).