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. Author manuscript; available in PMC: 2011 Feb 23.
Published in final edited form as: ACS Nano. 2010 Feb 23;4(2):625–631. doi: 10.1021/nn901319y

Figure 2.

Figure 2

Fluorescence of alexa 488 Streptavidin probe on cells pretreated with various concentrations of Sulfo-NHS-LC-Biotin. Biotinylation of the cell membranes of Hela cells was accomplished by incubating cells with the respective concentration of sulfo-NHS-LC-Biotin in PBS for 20 min. The cells were then treated with 15 μg/ml Alexa 488 Streptavidin in optiMEM for 30 min on ice. The confocal images were obtained by focusing on the top of the cell membranes, except for the 0.067 mM condition where the microscope was focused on the bottom of cells due to weak signal at the top of cell membranes.