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Analysis of photolabeling of RnPGGTase II with DATFP-amide ([32P]3) by SDS-PAGE. Lanes 1 and 1′: PGGTase II and [32P]3, no UV irradiation. Lanes 2 and 2′: RnGGPTase II and [32P]3, 1 min UV irradiation. Lanes 3 and 3′: RnPGGTase II, [32P]3, and GGPP, 1 min UV irradiation. Lanes 1, 2, and 3 show the silver-stained proteins. Lanes 1′, 2′, and 3′ show the radiolabeled proteins.
