Figure 2.

A, Coomassie Blue-stained SDS-PAGE profile of bovine brain proteins captured and released from an LK-derivatized (baited) DADPA column or an unbaited, mock-derivatized column. M, Molecular weight markers; F₀, 20 μg of bovine brain lysate before affinity chromatography; Blk, last column volume of wash (running) buffer before LK elution; − and +, protein fractions eluted with 100 mm LK from the unbaited and baited columns, respectively. Numbers adjacent to bands indicate proteins that were subjected to tryptic digestion, extraction, and mass spectrometry. Protein identities of these bands are indicated in Table 1. B, C, The + and − protein fractions were immunoblotted using antibodies against LanCL1 or DRP2/CRMP2 as an orthogonal method of MS corroboration. WB, Western blot.