Table 1.
Mass spectrometric identification of proteins eluted from mock- and LK-baited columns
| Band(s) | Identity | Predicted pI | MOWSE |
|---|---|---|---|
| 1, 3 (−) | Nucleolin-related protein-1 (NRP-1) | 4.8 | 129 |
| 2 (−) | Valosin-containing protein | 5.3 | 745 |
| 4 (−) | Nucleosome assembly protein-4 (NAP-4) | 5.1 | 249 |
| 5 (−) | β-tubulin | 4.9 | 250 |
| 6 (−) | SET translocation protein | 4.2 | 403 |
| 7 (−) | Acidic nucleophosphoprotein-2 | 5.1 | 160 |
| 8 (−) | Casein kinase-IIβ | 5.6 | 136 |
| 9 (−) | Ribosomal protein L22 | 9.8 | 402 |
| 10 (−) | Acidic 60S ribosomal protein-2 | 5.9 | 484 |
| 11 (+) | Complement H factor-1 | 6.8 | 151 |
| 12 (+) | Heat shock protein-70 (HSP-70) | 5.9 | 403 |
| 13 (+) | Collapsin response mediator-2 (CRMP2) [dihydropyrimidinase-like 2 (DRP2)] | 7.3 | 122 |
| 14 (+) | Syntaxin-binding protein-1 (Munc18-1, STXBP1) | 7.0 | 419 |
| 15 (+) | β-tubulin | 4.9 | 350 |
| 16 (+) | β-actin | 5.6 | 182 |
| 17 (+) | Lanthionine synthetase-like protein-1 (LanCL1) | 7.8 | 376 |
| 18 (+) | Myelin proteolipid protein (PLP) | 8.4 | 197 |
| 19–21 (+) | Myelin basic protein (MBP) | 11.3 | 290 |
“Band number” refers to protein band annotations in Figure 1; (−) and (+) indicate elution from unbaited and LK-baited columns, respectively. MOWSE, Molecular weight search.