Figure 1.
Sarcosine evokes a dose-dependent, Cl- current. (A) Chemical structures for sarcosine and glycine. (B) Currents from one neuron evoked by the indicated sarcosine concentrations. (C) Mean peak current elicited by each sarcosine concentration normalized to the peak current elicited by 10 mM sarcosine in the same cell (n = 5-11). Line shows the fit to a logistic equation with an EC50 of 3.2 mM and an N of 1.5. Data were obtained at -55 mV and -65 mV and were combined because they were indistinguishable. (D) Ramp IV curves from a neuron before applying sarcosine and during a 3 mM sarcosine application. Using the CsMeSO3 pipette solution, a neuron was held at 0 mV and subjected to voltage ramps from -100 to +40 mV. Complete current trace from which the ramp IV curve during a 3 mM sarcosine application was taken (Inset).