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Phenotyping is straightforward as zebrafish larvae are transparent
(a), Phenotype at 48 hpf of an embryo injected with mismatch control oligo compared with a morphant (injected with 25 ng of morpholino dsc2) embryo at the same stage (b). High-resolution pictures of the heart of morphant embryo (c) compared to control-injected embryo (d). Transmission electron microscopy of cardiac desmosomes from a control embryo (e) and morphant embryo (f) at 48 hpf.
