Figure 6.

The ion count at 616 m/z corresponding to the dissociated heme groups (top) and the background subtracted total ion count (bottom) for a 2 minute segment of the continuous lysis/CE-MS experiment. The dissociated heme groups migrated faster through the separation channel, so each lysis event generated a 616 m/z peak approximately 10 seconds before the major peak (visible in the TIC) corresponding to the α and β hemoglobin subunits. The peaks corresponding to the first 4 cell lysis events observed in this detection window are labeled in both traces to help illustrate the trend which continues throughout the entire run. The gray rectangles correspond to the data that was used to generate the spectra labeled with the same letters in figure 7.