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. 2010 Mar 15;21(6):1047–1058. doi: 10.1091/mbc.E09-11-0944

Figure 2.

Figure 2.

The ERAD of ENaC subunits is dependent on both the Hrd1 and Doa10 ubiquitin ligases. Cycloheximide chase reactions were performed as described in Materials and Methods in HRD1/DOA10 (●), hrd1Δ (▴), doa10Δ (▵), or hrd1Δdoa10Δ (○) yeast strains (Pagant et al., 2007) expressing C-terminally HA-tagged α-, β-, or γ-ENaC. Chase reactions were performed at 37°C, lysates were immunoblotted with anti-HA (ENaC) or with anti-Sec61 (as a loading control). Data represent the means of 4–6 experiments, ±SEM.