Figure 1.

Interactions between Vps4 and its substrate and regulators. (A) Vps4 interaction network based on previous studies (Scott et al., 2005b; Azmi et al., 2008; Kieffer et al., 2008; Bajorek et al., 2009a; Xiao et al., 2009). (B) Model for the recruitment and assembly of Vps4. The numbers indicating New Interactions or Lost Interactions refer to the numbers in A. (C) Alignments of the putative MIM1 and MIM2 motifs of yeast and mammalian ESCRT-III subunits (yeast Ist1 does not contain an obvious MIM2 consensus sequence). Mutations used in this study are marked in red.