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. 2010 Mar 15;21(6):914–925. doi: 10.1091/mbc.E09-10-0901

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Figure 3. — Cdh1-resistant CIN8 does not promote bipolar spindle assembly in CDH1-m11 cells. (A) Fluorescence microscopy of cells with MYC-CIN8 or Cdh1-resistant MYC-CIN8-ak (coding for Myc-Cin8-alaKEN, with KEN box residues mutated to alanine), 60 min after release from α-factor block. Bars, 5 μm. (B) Quantification of SPB separation of cells from A at indicated time points from α-factor release. (C) Immunoblots of released cells. Clb2 and exogenous HA-Acm1 are degraded normally. Cin8-ak is resistant to Cdh1-m11–mediated proteolysis.