CDH1-m11 cells accumulate Pds1 but not Cdc20, and lengthen their spindles upon cohesin inactivation. (A) Left, immunoblots against strains synchronously released from α-factor with endogenously tagged Cdc20 and either CDH1 or CDH1-m11. Right, quantification of normalized Myc-Cdc20 levels from immunoblots. (B) Left, immunoblots against strains synchronously released from α-factor with endogenously tagged Pds1 and either CDH1 or CDH1-m11. Right, quantification of Myc-Pds1 levels standardized to Pgk1 loading control. (C) Average distance between separated SPBs in CDH1-m11 SCC1 and temperature-sensitive CDH1-m11 scc1-73 cells, 2 h after release from α-factor block. (D) Histogram of distance between SPBs from C.