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Rad17/9-1-1 complex but not phospho-Ser373 of Rad9 is required for the checkpoint-related accumulation of TopBP1 at stalled replication forks. Mock-depleted extracts (lane 1 and 2) and 9-1-1–depleted extracts supplemented with buffer alone (lane 3), r9-1-1-WT (lane 4), or r9-1-1-S373A (lane 5) were incubated with sperm chromatin in the absence (lane 1) or presence of aphidicolin (lanes 2–5). Chromatin fractions were isolated and immunoblotted with the indicated antibodies.
