Figure 2.

PHB-2 and CHCHD2 (Har-1) mutants localize to mitochondria. Hela cells were transfected with plasmid expression vectors expressing myc-tagged human PHB-2, human PHB-2(E130K), CHCHD2 (the human ortholog of Har-1), and CHCHD2(G65E). After 18 h cells were stained with MitoTracker Red, fixed, and stained with anti-myc antibody (green). PHB2 is known to localize to and CHCHD2 suspected of localizing to mitochondria. Each of the mutants also colocalized with the MitoTracker Red. In some cells such as the one shown for PHB-2 wild type, the myc-staining pattern resembled MitoTracker staining, but no MitoTracker was visible in the cells.