Figure 2.
Myo1d and IAP partially colocalize at tips of microvilli. Frozen sections of mouse small intestine labeled with antibodies targeting Myo1d (green) and IAP (red). (A) Confocal image of a villus demonstrating both Myo1d and IAP localize to microvillar tips. (B) Representative view of partial colocalization between Myo1d and IAP at microvillar tips. Myo1d at microvillar tips appears in two populations: alone in distinct puncta, and colocalized with IAP. Arrowheads mark IAP-enriched puncta that lack Myo1d at the extreme distal tips of microvilli. Bars, (A and B) 20 μm; inset in B, 5 μm.