Table 2.
Summary of FRAP kinetic data
Construct | Afast | kfast (s−1) | Aslow | kslow (s−1) | Afast/Aslow | Mobile fraction (α) |
---|---|---|---|---|---|---|
EGFP-Myo1a (n = 14) | 0.12 ± 0.04 | 0.34 ± 0.14 | 0.66 ± 0.04 | 0.06 ± 0.01 | 0.18 | 0.78 ± 0.01 |
EGFP-Myo1d (n = 22) | 0.38 ± 0.05 | 0.34 ± 0.05 | 0.55 ± 0.04 | 0.06 ± 0.01 | 0.69 | 0.93 ± 0.01 |
Photobleaching recovery curves were fit to a kinetic model as described in Materials and Methods. Values listed here represent fit parameters ± SE of the fit; Ax, amplitude for process x; kx, rate for process x; and n, number of BBs sampled.