Table 3.

Cell cycle timing of contractile ring assembly andconstriction

Strain	Time relative to spindle breakdown (min)a
	Assembly complete	Constriction initiates
Wild typeb	−8.3 ± 2.7	+5.7 ± 2.0
cdc8-27	−1.3 ± 2.9	+8.9 ± 3.3
myo2-E1b	+5.6 ± 6.2	+15.4 ± 6.7
2xYFP-myo2	−14.0 ± 3.2	+6.7 ± 2.9
cdc8-27 2xYFP-myo2	−14.1 ± 5.2	+8.7 ± 4.0

^a Values represent the mean (±SD) generated from analysis of rings and SPBs in 30 cells. Values correspond to the average datasets provided in Figure 5C. Spindle breakdown is defined as the time when SPB separation peaks.

^b Contractile rings in these strains visualized by Rlc1p-GFP.

All cells were grown at 25°C, and time-lapse microscopy was carried out at room temperature.

The differences in constriction initiation times in wild-type vs. mutants were significant (p = 0.0006 for cdc8-27; p = < 0.0001 for myo2-E1).