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Overexpression of the thioredoxin system reduces glutathionylation. The wild-type strain containing pRS426 (v), thioredoxin system (Trx1 and Trr1) or glutaredoxin system (Grx1 and Glr1) was grown to exponential phase in minimal SD media. Reduced (GSH), oxidized (GSSG) and protein bound (GSSP) glutathione levels were determined. Values shown are the means of at least three independent determinations and are given in nmol/ml/A600.
